グルタチオン増加物質のスクリーニング方法

Procede de criblage pour obtenir une substance capable d'augmenter le taux de glutathion

Method for screening of substance capable of increasing glutathione

Abstract

神経変性疾患、悪性腫瘍、感染症などの疾患治療手段として、グルタチオンを増加させる治療薬の有効成分となる物質をスクリーニングするための手段を提供する。この方法では、GTRAP3-18蛋白質を発現する細胞に被験物質を接触させ、GTRAP3-18蛋白質の発現量を減少させるか、またはGTRAP3-18蛋白質の機能を抑制する物質を、目的物質として特定する。
Disclosed is a method for screening a substance which can increase the glutathione level and can be used as an active ingredient for a therapeutic agent, as a means for treatment of a disease such as neurodegenerative disease, malignant tumor and an inflammatory disease. In the method, a candidate substance is contacted with a cell capable of expressing GTRAP3-18 protein, and the candidate substance is determined to be a desired substance when the candidate substance decreases the GTRAP3-18 protein expression level or suppresses the function of GTRAP3-18 protein.
La présente invention concerne un procédé de criblage pour obtenir une substance qui est capable d'augmenter le taux de glutathion et qui peut être utilisée en tant que matière active d'un agent thérapeutique destiné au traitement d'une maladie telle qu'une maladie neurodégénérative, une tumeur maligne ou une maladie inflammatoire. Dans ledit procédé, une substance candidate est mise au contact d'une cellule capable d'exprimer la protéine GTRAP3-18, la substance candidate étant déterminée comme étant l'une des substances que l'on recherche lorsque la substance candidate diminue le taux d'expression de la protéine GTRAP3-18 ou qu'elle inhibe la fonction de la protéine GTRAP3-18.

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